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KMID : 0378019630060100063
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New Medical Journal
1963 Volume.6 No. 10 p.63 ~ p.78
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Study on Inflammation0Inducing Ability of the Extract of the Normal and Necrotic Tissues.
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Abstract
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Since it is revealed that, as discussed in previous paper, there exist certain active substances in both normal and necrotic tissues which may set up inflammatory reaction, the author undertook another experiment toistudy,further the nature of inflammation-inducing substances. Although inflammatory ability was demonstrated in the extract of tissues of various organs, liver and kidney specimens only were selected as material, bcause they had moderate degree of inflammation-inducing ability. Normal and necrotic tissue extracts were prepared ;in the same manner as in the previous experiment, but this time they were analyzed by some chemical procedures and were fractionated by paper electrophoresis in order to isolate the active substances from them. The different substances thus obtained were injected intracutaneously into the abdomen of normal rabbits, and microscopic examination of those lesions was made at intervals of 6 hours, 12 hours and 24 hours, with following results:
1) Inflammation-inducing substances existing in the extracts of normal and necrotic tissues are shown to have a property not to be solved by such fat solvents as chloroform and alcohol, but rather to be precipitated almost completely by aceton and trichloracetic acid.
2) Inflammation-inducing substances belong, for the most part, to ~-globulin fraction by their nature, but it is believed that they are somewhat present even in albumin, al-globulin, a2-globulin and 7--globulin fractions.
3) Necrotic tissue substances which belong to /3-globulin 4raction show, as a rule, a more ;powerful inflammatory reaction than those of normal tissue.
4) Substances chiefly concerned with inflammation-inducing ability of both normal and necrotic tissues are thought to belong to ~-globulin fraction, or ~-globulin like substances.
From a series of part I and II experiments, conclusions have been summarized as follows:
1) One of the most important and significant endogenous materials concerned with inflammatory mechanism could be stated to be a constituent of normal cells which belong to 7~-globulin fraction by paper electrophoresis.
2) It seems that normal tissue cells, if subjected to injury, may release some inflammation-inducing substances from them, thus causing inflammatory reaction for protection of the individual from the injury.
3) 6-globulin fraction of necrotic tissue is considered to have a more powerful ability in causing inflam. matory reaction than that of normal tissue, and this seems to be of some help to account for the fact that the more destructive an inflammatory lesion is, the more marked inflammatory reaction usually takes place.
4) The author would like to state on his own view of inflammation formed from the result of these extensive studies that inflammation is induced by release of (inflammation-inducing substances from tissue cells, subjected to injury, and it is further prompted ;by action of such various endogenous substances, as Histamine, Histamine-like substance, Leukotaxine, and Necrosin which were all demonstrated in inflammatory exudates.
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